Nanotechnology Used to Enter Plant Cells

US scientists are using nanotechnology to penetrate plant cell walls and deliver a gene and a chemical triggers with great precision. The work could lead to a powerful new tool for targeted delivery into plant cells.

The research is highlighted in the May issue of Nature Nanotechnology. Kan Wang of Iowa State University and his colleagues point out that introducing a gene into a plant cell is possible but chemical activation usuall involves an imprecise and separate process that may be toxic to the plant.

“With the mesoporous nanoparticles, we can deliver two biogenic species at the same time,” Wang said. “We can bring in a gene and induce it in a controlled manner at the same time and at the same location. That’s never been done before.”

The controlled release will improve the ability to study gene function in plants. And in the future, scientists could use the new technology to deliver imaging agents or chemicals inside cell walls. This would provide plant biologists with a window into intracellular events.

Lin’s porous, silica nanosphere system has arrays of independent porous channels, which form a honeycomb-like structure that can be filled with chemicals. “One gram of this kind of material can have a total surface area of a football field, making it possible to carry a large payload,” Trewyn said.

Nanotech threat to your safety

Nanotechnology cartoon - Scott Dougherty, LLNLDoes nanotechnology pose a threat to humanity on a par with the threat we face from genetically modified (GM) foods? That is the question asked by various lobbying groups from the verdant greens to the confused consumers. But, governments, academics and commercial bodies see the issue from an entirely different perspective and are asking, will the nanotechnology industry face the same outpouring of hostility as that caused by genetically modified (GM) foods? The kind of outpouring that stymies and often destroys research that could benefit us all.

Nanotechnology, the application of objects and structures that are very small, usually less than 100 nm in diameter is growing rapidly. But aside from the bizarre imaginings of those who originally pioneered the term, this new science is really not much different from what chemists, materials, scientists, and physicists have always done and that is to work with particles, molecules, and other species that just happen to be individually very small. The advent of scanning microscopy techniques has given us a closer look at the structure of atomic clusters, supermolecules and nanomaterials, and new techniques for handling materials at the very small scale or creating species that have novel functionality at this level are emerging all the time. However, the nano, meaning a billionth (in this case a billionth of a metre) is really nothing new, it simply defines the size limits.

Much of the nano that hits the press is nothing of the sort, remember those little cogs and pistons etched in silicon? And, what about the tiny bots that would defur arteries? They are a thousand times bigger than truly nanoscopic objects and the likes of liposomes, which the cosmetic industry briefly marketed as nanotechnology are really nothing of the sort, they are simply supramolecular chemistry given a trendy name.

According to a Leicester University press release this week touting a talk to be
given in May by new media expert Rachel Gibson is studying the nature of the growing online nanotech debate and will present her latest findings to the annual meeting of the International Communication Association in May 2007. The release says that, “at such scales, the ordinary rules of physics and chemistry no longer apply.” This is baloney! The normal rules of physics and chemistry very much apply, at almost every scale. In fact it is at the nanoscale that we can see the beauty of the normal rules of physics and chemistry in action – the quantum effects, such as tunnelling, the formation of electrostatic, non-covalent bonds, the non-linear behaviour that continually astounds but never breaks those laws. These are the normal rules of the physical sciences. There is no mystery about nanotechnology.

That said, the promise of nanotechnology is as big as the grants researchers are receiving should they happen to splash a few “nanos” into the application forms. Novel materials and composites indeed do have huge potential medicine, engineering, communications, transport, even the home.

But, the inevitable questions from environmental groups and ethicists are repeatedly raised. The outpourings of certain British Royals concerning “grey goo” and other garbage do not lend themselves to a sensible debate either. And, yes, there should be a debate if we are finding such unique physical behaviour on this scale that it represents some kind of threat. But, the idea of self-replicating nanobots that chomp their way across the planet, is quite literally, a Michael Crichton plot, I believe, rather than a serious prediction about where nano is heading.

Gibson, however, hopes to examine the efforts of the scientific community to engage with the various social concerns and to assess the extent to which the GM lessons are being learned as nanotechnology (more realistically at the moment mere nanoscience) diffuses into the public consciousness.

She is working with colleagues from the Australian National University, to develop software to study the structure, evolution and implications of the online hyperlinking activities of political and social organisations. The Virtual Observatory for the Study of Online Networks (VOSON) (http://voson.anu.edu.au) thus provides social scientists with the means to study the success of opposition of groups. Watching the evolution of the debate online offers a new way of studying this question, she says, particularly as many of the groups active on the issue are enthusiastic users of the Web.

“This project demonstrates the growing interest among social scientists in applying online technologies, and particularly cyber-mapping tools, to address important social science questions,” she says, “The research allows us to examine the development and expansion of issue networks in a wholly new three dimensional space that means we can track the formation of alliances between groups over time and across countries.”

One cannot help but wonder whether all the money being spent on such endeavours, the cash ploughed into lobbying, and the cost of endlessly debating non-issues, could not be better spent by the nanoscientists themselves to investigate with even greater precision the properties and safety aspects of their discoveries. Surely, the money could assist the maturation of nano, rather than us having to see scientists fending off lobbyists and rebuilding labs, as opposed to GM crops, trashed by extremists.

Build a better mousetrap, they say, and the world will beat a path to your door. While, nano is not really anything but a question of scale, it really could be the better mousetrap we have been looking for, figuratively speaking. Advocates have to hope that those beating at their door are as keen to see the technology mature safely as they are.

Knights of the chemical realm

Right royal honours were bestowed on two of my very oldest contacts in the field of chemistry this week. Royal Society of Chemistry president Professor Jim Feast was awarded the CBE in the New Year Honours List for ‘services to polymer chemistry.’ And, supramolecular ex-pat Fraser Stoddart was given a Knighthood for to ‘services to chemistry and molecular nanotechnology.’

Congratulations to them both. With such accolades under their belts, it’s surely only a matter of time before either one of them gets that phone call from Stockholm too.

Buying Nano This Holiday

According to the Nanotech Project, more than twenty years of research, has begun to yield the first commercial applications for nanotechnology in consumer products. They explain that nanoscale materials can now be found in electronics, cosmetics, automotive parts, and medical products. Apparently, there are about 350 such products.

But, while the British media is running scared at the idea of nanotechnology turning the world to grey goo (and we’re not talking Second Life here), The Register reports on a Nature Nanotechnology paper that claims almost three quarters of Americans have heard little or nothing about nanotechnology. Nano, of course, refers only to the scale of any particular particle or component of any technology.

Nano, meaning nothing more than a billionth, so a nanometre is a billionth of a metre. Funnily enough, most of the so-called nanoscale products actually have dimensions of several hundred nanometres, so in truth such objects could be called microscale (or in more familiar parlance microscopic). Micro meaning a millionth. A micrometre is therefore a millionth of a metre. Something that’s 500 nanometres might just as readily be described as half a micrometre.

The author of the Nature Nanotechnology paper warns that public ignorance in the US of nanotechnology means that legislation may be passed without informed debate. Fair point, but it can be pushed to the opposite extreme with precautionary attitudes predominating and stifling research and development that might benefit us all, as has happened in the UK with respect to genetically modified organisms (GMOs and GM food).

Do you agree?

Totally tubular peptide rings

peptide nanotubes

The highly unique crystal structure of nanotubes constructed from cyclic peptides is revealed this month by Japanese researchers in the journal Organic Biomolecular Chemistry. The descendents of these novel nanotubes could find a role in future molecular electronic devices, according to the team, who allude to the high macrodipole moment of their materials.

Shunsaku Kimura and colleagues at Kyoto University, have built on the work of ETH’s Dieter Seebach and Wisconsin’s Sam Gellman to use supramolecular chemistry to construct through self-assembly a stacked column of cyclic peptides, themselves made from three ACHC amino acids linked in a ring. ACHC is the trans-2-aminocyclohexylcarboxylic acid. The team used Fourier transform infra-red and nuclear magnetic resonance spectroscopy measurements and computational calculations to demonstrate that this cyclic tri-beta-peptide has C3-symmetry with the amide groups in the trans positions.

To read my full article visit the spectroscopyNOW.com crystallography channel.

Nanotech Pioneers

The Nanotech PioneersIt is on the nanometre scale where chemists, physicists, materials scientists and engineers, and even biologists will meet to create a new technology – nanotechnology. This book’s cover claims nanotech was “scarcely imagined a few decades ago”, but what about Fantastic Voyage, and, of course, Feynman’s predictions? Well, like they say, don’t judge a book by its cover. Nanotech is likely to dominate the 21st century and affect our lives in ways we have not yet determined. By definition, nanotech is far too small to be visible to the human eye, and so its effects may well catch us by surprise.

Despite nanotech’s science fiction aura, consumer products already exist that rely on its earliest manifestations, although one has to say that most of these are not the nanotech of molecular machines, but generally just particles that are nanometres across or materials with nanoscale features that endow them with their particular properties.

The variety of new products and technologies that will spin out of nanoscience will, nevertheless be limited only by the imagination of the scientists, engineers and entrepreneurs drawn to this new field – The Nanotech Pioneers.

Steve Edwards presents nanotechnology and its leading makers in an easily understandable fashion, suited for all readers regardless of academic background, but with enough facts and details to separate the hype from the real nanotech that is just around the next corner. Edwards brings nanotechnology closer to the science-interested general public as well as scientists, students, organizations, journalists, politicians, and entrepreneurs.

Buckyball co-discoverer dead at 62

It is with sadness that we report the death of nanotech pioneer, Richard Smalley at 62. Smalley, along with Harry Kroto and colleagues discovered the all-carbon fullerene molecule in the early 1990s at a time when I was just beginning my career as a science writer. Their fascinating research into the soccerball shaped molecules, which were nicknamed buckyballs by the popular science press, provided many of us with some of the greatest punning opportunities ever in science. More seriously, they and their tubular offspring are after many years of detailed and fundamental research beginning to reach new goals in the field of nanotechnology.

Smalley undertook pathbreaking research, showed an incredible commitment to teaching, and was dedicated to the idea of betterment of our world.

He died in Houston on 28th October after a long battle with cancer.